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KMID : 0352720170410040608
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Journal of Ginseng Research
2017 Volume.41 No. 4 p.608 ~ p.614
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Ginsenoside Rg1 from Panax ginseng enhances myoblast differentiation and myotube growth
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Go Ga-Yeon
Lee Sang-Jin
Jo A-Young
Lee Jae-Cheol
Seo Dong-Wan
Kang Jong-Sun
Kim Si-Kwan
Kim Su-Nam
Kim Yong-Kee
Bae Gyu-Un
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Abstract
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Background: Ginsenoside Rg1 belongs to protopanaxatriol-type ginsenosides and has diverse pharmacological activities. In this report, we investigated whether Rg1 could upregulate muscular stem cell differentiation and muscle growth.
Methods: C2C12 myoblasts, MyoD-transfected 10T1/2 embryonic fibroblasts, and HEK293T cells were treated with Rg1 and differentiated for 2 d, subjected to immunoblotting, immunocytochemistry, or immunoprecipitation.
Results: Rg1 activated promyogenic kinases, p38MAPK (mitogen-activated protein kinase) and Akt signaling, that in turn promote the heterodimerization with MyoD and E proteins, resulting in enhancing myogenic differentiation. Through the activation of Akt/mammalian target of rapamycin pathway, Rg1 induced myotube growth and prevented dexamethasone-induced myotube atrophy. Furthermore, Rg1 increased MyoD-dependent myogenic conversion of fibroblast.
Conclusion: Rg1 upregulates promyogenic kinases, especially Akt, resulting in improvement of myoblast differentiation and myotube growth.
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KEYWORD
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ginsenoside Rg1, myoblast differentiation, myogenic conversion, myotube hypertrophy, promyogenic signaling
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